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Bisbenzimide (Hoechst 33342) is an organic compound used as a fluorescent stain for DNA in molecular biology applications. Several related chemical compounds are used for similar purposes and are collectively called Hoechst stains.
Video Bisbenzimide
Application
Bisbenzimide tends to bind to A-T rich regions of DNA and can decrease its density. Bisbenzimide mixed with DNA samples can then be used to separate DNA according to their AT percentage using a cesium chloride (CsCl) gradient centrifugation.
Maps Bisbenzimide
References
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External links
- Fluorescence Spectra: http://www.fluorophores.tugraz.at/substance/463
Source of the article : Wikipedia
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